HiYield™ Plasmid Large DNA Mini Kit is designed for rapid isolation of up to 30 £gg of 10-50 kb plasmid or cosmid DNA from 1-4 ml of bacterial cultures within 15 minutes without phenol extraction and alcohol precipitation. Typical yields are 20-30 £gg for high-copy plasmid or 3-10 £gg for low-copy plasmid from 4ml of bacterial cultures. Purified DNA is ready for direct use in DNA Sequencing, DNA Library Screening, Restriction Enzyme Digestion, PCR, Ligation and Transformation.
AmpEasy™ Direct RT-PCR Kit is designed for high-speed preparation of first-strand cDNA directly from cultured cells without RNA purification. By using AmpEasy™ Direct RT-PCR Kit, RNA in the cell lysate can be directly converted to cDNA and subsequently analyzed using the PCR reagents included in the kit. The whole process, from cells to cDNA and from cDNA to PCR, can be completed in less than 1.5 hours.
AmpEasy™ Cells cDNA Kit is designed for high-speed preparation of first-strand cDNA directly from cultured cells without RNA purification. By using AmpEasy™ Cells cDNA Kit, RNA in the cell lysate can be directly converted to cDNA. The whole process, from cells to cDNA, can be completed in less than 30 minutes. When AmpEasy™ Cells cDNA Kit is used together with optimized PairFast™ Real-Time PCR Mastermix for real-time PCR, several cell samples can be easily processed and analyzed within a few hours.
AmpEasy™ Plant Direct PCR Kit is designed for performing PCR directly from plant leaf tissues without DNA purification or sample preparation. AmpEasy™ Plant Direct PCR Kit has been validated for the direct amplification of DNA fragments from leaves and seeds from a wide variety of plant species. Fresh plants, plant material stored at +4¢XC or frozen at -20¢XC are all suitable templates for this kit, as well as plant material stored on commercially available cards such as Whatman 903 and FTA Cards.
AmpEasy™ Blood Direct PCR Kit is designed for performing PCR directly from whole blood without DNA purification or sample preparation. AmpEasy™ Blood Direct PCR Kit has been validated for the direct amplification of DNA fragments from whole blood, blood collected in different anti-coagulant tubes(such as EDTA, citrate or heparin), on FTAR Elute Cards, Whatman 903R Specimen Collection Paper (¡§Guthrie cards¡¨) or regular filter paper.
HiYield Plus™ PCR Purification Kit is designed to rapid recover or concentrate DNA fragments (70 bp-20 kb) from PCR or other enzymatic reactions within 10 minutes. pH indicator and ready-to-use 3M Sodium Acetate (pH5.0) buffer are provided. pH Indicator provides visual identification of optimal pH, ensuring maximum yields. Typical recovery is up to 95%. The purified DNA is ready for use in many downstream applications.
HiYield Plus™ Gel Extraction Kit is designed to rapid recover or concentrate DNA fragments (70 bp-20 kb) from agarose gels within 20 minutes. QGE Buffer contains an integrated pH Indicator which provides visual identification of optimal pH, ensuring maximum yields. Typical recovery is around 90%. The purified DNA is ready for use in many downstream applications.
HiYield™ Total RNA Mini Kit (Yeast) is ideal for purification of total RNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species in a simple spin column format. Sorbitol Buffer is included in the kit to reduce sample preparation time. Up to 30 £gg of total RNA can be purified within 20 minutes without phenol/chloroform extraction or alcohol precipitation. Typical yield is around 20 £gg for 5 x 107 of Saccharomyces cerevisiae.
HiYield™ Genomic DNA Micro Kit provides a fast and economical method for purification of genomic and mitochondrial DNA from small volumes of whole blood, dried blood spots and urine. The entire procedure can be completed in 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 300 ng from 6 mm diameter of blood spots and with a minimum DNA yield of 300 ng from 10 £gl of whole blood.
HiYield™ Genomic DNA Extraction Kit (Buccal Swab) provides a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from buccal cells within 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 2 £gg per buccal swab. The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis.